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. 2019 Mar 19;9:4853. doi: 10.1038/s41598-019-41269-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental autoimmune encephalomyelitis (EAE) is enhanced in Anp32b KO mice. (a) Representative disease course in Anp32b KO (n = 31) and Anp32b wild type (WT) mice (n = 26) after immunization with MOG peptide emulsified in CFA and enriched with M. tuberculosis. Clinical score measurement was performed to indicate the onset, severity and duration of the autoimmune disorder. (b) Isolated splenocytes from Anp32b KO and WT animals that had experienced 30 days of EAE were restimulated with MOG peptide at the indicated concentrations. Cell proliferation was subsequently measured after 4 days of restimulation by incorporation of ^[3H]methyl-thymidine. (c) Supernatants from (b) were analyzed for expression of IL-17A, TNF-α, and IFN-γ and IL-21. Data in panel (a–c) are mean ± SEM. EAE scoring significances: days 6, 8, 14, 15, 16, 19, 21, 26, 29 (NS); days 12 (p < 0.05); day 30, 55, 60 (p < 0.01) and day 34, 41, 47 (p < 0.001). Pooled data from two independent experiments are shown. *p < 0.05, **p < 0.01, ***p < 0.001, Mann–Whitney U test (a), two-way ANOVA (b), Student t test (c).