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. 2019 Mar 19;9:4868. doi: 10.1038/s41598-019-41057-9

Figure 3.

Figure 3

TLP is required for etoposide-induced transcriptional repression. (a,b) The rate of ongoing transcription was analyzed by metabolic labeling of newly transcribed RNA using 4sU. 4sU-RNA labeling was performed using control (ctrl) and TLP-knockdown (shTLP) HeLa cells that were treated with 50 μM etoposide for indicated times, and the total quantity of 4sU-labeled RNA was measured with a Quantus fluorometer (a). qRT-PCR was performed using 4sU-labeled RNA as a template (b). Data were normalized to the level of non-treated cells (0 h) and represent the average and S.D. of three independent experiments. *p < 0.05; **p < 0.01; n.s, non-significant. (c) Control and TLP-knockdown HeLa cells were treated with DMSO or 50 μM etoposide for 24 h, and ChIP was performed using anti-RNAPII CTD. Data were normalized to the level of DMSO-treated conditions and represent the average and S.D. of three independent experiments. *p < 0.05, n.s., non-significant.