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. 2019 Mar 19;9:4851. doi: 10.1038/s41598-019-41311-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H₂O₂ induces apoptosis in DEF cells. (A) Cells were observed using phase contrast microscopy (×100). Non-H₂O₂-treated cells were used as controls (B). The cells were fixed and stained with DAPI. The arrows indicate apoptotic nuclei, and non-H₂O₂-treated cells were used as controls. (C) TUNEL assay. The arrows refer to TUNEL- positive cells, and non-H₂O₂-treated cells were used as controls.