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. 2019 Mar 19;9:4851. doi: 10.1038/s41598-019-41311-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activity of caspase-3 and caspase-9 induced by H₂O₂ in DEF cells. (A) The mRNA expression levels of caspase-3 and caspase-9 in H₂O₂-treated and non-H₂O₂-treated cells. (B) The activity of caspase-3/7 and caspase-9 in H₂O₂-treated and non-H₂O₂-treated cells. All data are presented as the means ± SD from three individual experiments. Non-H₂O₂-treated cells were used as controls. p* < 0.05 compared to the non-H₂O₂-treated controls. (C) Flow Cytometry results indicated that H₂O₂-treated cells have 22% apoptosis cells.