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. 2019 Mar 19;9:4851. doi: 10.1038/s41598-019-41311-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of the Us5 protein in DEF cells. (A) Indirect immunofluorescence of DPV Us5 in DEF cells. Costaining was performed with a rabbit anti-DPV-Us5 polyclonal antibody and a fluorescein-conjugated goat anti-rabbit IgG antibody. (B) Western blotting of DPV Us5 in DEF cells. A rabbit anti-DPV-Us5 polyclonal antibody was used as the primary antibody. A goat anti-rabbit IgG (H + L)-HRP antibody was used as the secondary antibody. Noninfected cells were used as controls. (C) Western blotting of pCAGGS-Us5 in DEF cells. A duck anti-DPV polyclonal antibody was used as the primary antibody. A goat anti-duck IgG (H + L)-HRP antibody was used as the secondary antibody. Cells transfected with the pCAGGS plasmid were used as the controls.