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. 2019 Mar 19;9:4842. doi: 10.1038/s41598-019-41008-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth inhibition in the absence and presence of INH (A) and RIF (B). Mycobacterial growth was assessed in titration curves. INH inhibited mycobacterial growth in a dose-dependent manner in the naïve and vaccinated groups (A,B), as well as RIF (D,E). Data from both groups was compiled in dose-response box plots to identify the BCG effect in addition to the INH- and RIF-mediated killing (C,F). Dots and squares in the titration curves (A,B,D,E) represent individual data points from the participants and the central lines indicate the median response with IQR. Each group is represented in a single box plot with range in the dose-response analysis (C and F). Samples size is indicated in the figure (for INH: n = 29 BCG-vaccinated and n = 21 BCG-naïve participants; for RIF: n = 25 BCG-vaccinated and n = 18 BCG-naïve participants). Statistical significances were tested using one-way ANOVA (A,B,D,E) and unpaired t-test (C,F).