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. 2019 Mar 19;9:4842. doi: 10.1038/s41598-019-41008-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Frequencies of Th1 cytokine-expressing lymphocytes. Expressions were measured from PBMCs after stimulation with BCG, with and without 1 μg/ml of INH, for 4 days. The grey dot symbols represent stimulation with BCG only, while the black squares represent BCG + INH. Comparisons were made between the naïve (blue) and historically BCG-vaccinated (red) groups. Data is presented as percentages of cell population of interest, and displayed as bar graphs with error bars represent mean ± SD. For this experiment, PBMCs from an alternate cohort of participants were used (Table 1), consisted of 16 naïve and 34 historically BCG-vaccinated participants, with a comparable demographics profile and immune responses as the main cohort. The Mann-Whitney U test was used to determine significance. The P value < 0.05 is considered statistically significant.