Fig. 2. The circRNA17 may influence PCa proliferation and cell invasion via altering the ARv7.

a Knocking down circRNA17 in C4–2 parental cell results in decrease of Enz sensitivity. MTT assay was used to measure the cell proliferation in response to 10 µM Enz treatment in the C4–2 parental cells with a knockdown of circRNA17 (sh-circRNA17) or vector control (PLVTHM). b Knocking down circRNA17 in C4–2 parental cells increased cell invasion. Boyden chamber assay was used to measure the cell invasion in C4–2 parental cells with sh-circRNA17 or PLVTHM control cells. Quantitation is in lower panel. Scale bar, 10 µm. c The decrease of Enz sensitivity from knocking down circRNA17 can be reversed by a simultaneous knockdown of ARv7. MTT assay was performed as in a in cells with the indicated expression of various shRNA constructs. d The increase of cell invasion in transwell assay (upper) and 3D invasion (lower) assay as a result of sh-circRNA17 can be reversed by a simultaneous knockdown of ARv7 in C4–2 parental cells. Boyden chamber assay was performed in as in b in cells with the indicated shRNA constructs. Scale bar, 10 µm. e Knocking down PDLIM5 linear sequence outside the circRNA17 region in C4–2 and CWR22Rv1 (22Rv1) cells. f, g Knocking down PDLIM5 host gene linear sequence did not alter in C4–2 and 22Rv1 cells Enz sensitivity (f) and transwell invasion (g). Scale bar, 10 µm. For d and g, quantitations are at the right. *p < 0.05, **p < 0.01 and ns (or NS) = no statistical differences