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. 2019 Jan 15;10(2):37. doi: 10.1038/s41419-018-1048-1

Fig. 3. Enz can regulate circRNA17 expression in an AR-dependent manner.

Fig. 3

a Enz can repress circRNA17 expression in C4–2 parental cell with a concomitant increase of ARv7 at the transcript level. C4–2 parental cells were treated with 10 µM Enz for 48 h followed by the RNA isolation and measurement of circRNA17 and ARv7 with RT-qPCR. b Withdrawal of Enz in C4–2 EnzR cells results in an increase of circRNA17 as well as ARv7 at the transcript level. c Bioinformatic analysis of putative AR-binding sites in circRNA17 host gene PDLIM5 promoter. d ChIP assay indicates AR binds to ARE at position 876–884 upstream of PDLIM5 transcription start site. e, f Transfection of PDLIM5 5′ promoter constructs containing wild type or mutant ARE sequence into C4–2 and CWR22RV1 (22RV1) cells and luciferase assay was applied to detect the luciferase activity. For a, b, and f, relative normalized expressions are shown. *p < 0.05, **p < 0.01, ***p < 0.001 and ns=no statistical differences