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. 2019 Jan 24;28(3):296–305. doi: 10.1177/0963689718822773

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig 1. — Expanded PUC cells express urothelial stem/progenitor cell markers. (A) The representative images of PUC cells at passage 1 and 5 are shown. (B) Western blot analysis for CK5 and CK14 expression was performed on passage 3 PUC cells. (C) Immunofluorescence staining of passage 3 PUC cells was carried out with antibodies against CK5 (red) and CK14 (green), nuclei stained with DAPI, 400× magnification. Scale bars represent 50 µm.

DAPI: 4′6-diamidino-2-phenylindole; PUC: porcine urothelial cell.