Fig 2.

Cyclophosphamide-induced cystitis with changes of the urothelium and Intravesical instillation of PUC attenuates CPP-induced cystitis. The mice were intraperitoneally injected with 300 mg/kg CPP (300 mg/kg) and 106/100 μl, normal PUCs or vehicle control was intravesically instilled into the bladders 4 h after the CPP injection, and the bladder morphology, weight, H&E staining and bladder RNA extractions were performed after 24 h. (A) Representative images of the mouse bladders on day 1 after cyclophosphamide injection from the vehicle control and PUC-treated groups. Hemorrhage is evident. Note the obvious congestion, enlargement and hemorrhaging in the bladder of CPP-treated mice without intravesical PUC instillation. (B) Bladder weight/body weight ratio in the control and PUC-treated groups. (C) Representative histological changes the hematoxylin-eosin-stained bladder sections. Urothelium in the vehicle controls shows some remaining urothelial cells, and denuded areas. (D) Edema index of the bladder sections of vehicle control and PUC-treated mice. The data represent the mean ± SD of three independent experiments. Scale bars represent 50 µm. **P<0.01 versus the vehicle control.

CPP: cyclophosphamide; H&E: hematoxylin and eosin; PUC: porcine urothelial cell.