FIG 3.

Med1 is partially required for cell proliferation/survival. (A) Twin spot clonal analysis. Principle of generation of somatic clones in heterozygous wing imaginal discs, using FRT/Flp-induced recombination and the dorsal compartment-specific ap-GAL4 driver. The lower panel shows a model of wing disc under control conditions, where homozygous RFP^+/+ and GFP^+/+ twin clones display equivalent areas. (B) Scheme of the recombination event leading to the generation of mutant clones for the Med1^O2 allele marked with RFP associated with the wild-type twin clones marked with GFP. A representation of resulting clones is shown in the lower panel. (C) Principle of Med1^O2 mutant clone induction in a Minute (M) context (upper) with schematic representation of a resulting wing disc (lower). Note that Minute homozygous clones are lethal and do not proliferate. (D to D′′′) Wing disc bearing Med1^°2 mutant clones [RFP⁺ GFP⁻] generated as described for panel B. Magnifications of the zone of interest (D, dotted box) with overlay (D′) or single RFP (D″) or GFP (D′′′) channel are shown, and examples of mutant and control clones are surrounded by dashed lines. (E to E′′′) Wing disc bearing Med1^°2 clones generated in a Minute context as described for panel C, with higher magnifications in panels E′ to E′′′. An example of a Med1⁻ mutant clone is shown.