FIG 4.
Thoracic Med1 mutant clone phenotypes resemble GATA pnr loss of function. (A to C) Scanning electron microscopy images of Drosophila notum of wild-type (A), ap>flp; H2A-RFP Med1°2 FRT 80B/M ub-GFP FRT 80B (B), or pnr-GAL4MD37/pnrVX6 (C) flies. Filled arrows point to dorsocentral (DC) mechanosensory bristles, and open arrows point to thoracic clefts. (D and E) In situ hybridization against pnr mRNA in wild-type (D) versus Med1-deficient wing discs expressing dsRNA against Med1 (dsMed1) together with DCR2 in the dorsal wing disc of Med1Δ98/+ heterozygous flies (E). Disc areas giving rise to the presumptive thorax and corresponding to the Pnr expression domain are shown. (F and G) αMed1 immunolocalization in wild-type (F) versus Med1-deficient (G) wing discs, showing ubiquitous Med1 protein expression in the whole wing disc (F) and strong protein depletion in the dsRNA-expressing dorsal part (G).