FIG 6.

GATA/Pnr physically interacts with Med1. (A) Coimmunoprecipitation experiments from stable Drosophila lines expressing GFP-Pnr or GFP alone using anti-Med1 antibody or preimmune serum (control IP) revealed by Western blotting against Med1 or GFP tag. (B) Coimmunoprecipitation experiments from Drosophila S2 cells endogenously expressing pnr, using anti-Med1 antibody or preimmune serum. (C and D) GST pulldown binding assays of full-length ³⁵S-Pnr or protein fragments to GST-Med1 fusion (C) or to GST-Med1ex4 fusion (D) corresponding to the Med1^A-specific extension. The doublets observed with full-length Pnr or some truncated forms are due to alternative initiations at the regular or internal initiator AUG during the in vitro transcription/translation reaction.