FIG 7.
c-Fos promotes the miR-551a expression in breast cancer cell lines. (A) Bar graph showing relative increase in miR-551a expression by qPCR upon c-Fos transfection in a dose-dependent manner. c-Fos transfection was confirmed by Western blotting in MDA-MB-231 and ZR75 cell lines. (*, P < 0.05; **, P < 0.005; ***, P < 0.0005, by one-way analysis of variance) (B) Scatter plot showing the expression of c-Fos in terms of the threshold cycle (CT) value normalized to that of actin in breast tumors and adjacent normal breast tissues (n = 24; **, P = 0.0039, by an unpaired t test). (C) Proposed mechanism of action of miR-551a and targeting of FAK.