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. 2019 Feb 4;17(4):2443–2450. doi: 10.3892/etm.2019.7235

Figure 2.

Figure 2.

Detection of Slit2, Robo1 and Robo4 mRNA and protein expression in human CECs. (A) RT-PCR analysis yielded expected products at 176 bp and 226 bp for Slit2 and Robo4, respectively; no product for Robo1 was detected. ‘+’ indicates the addition of target-specific primers to the reaction and ‘-’ indicates the addition of no primers. (B-E) Immunocytochemistry analysis demonstrated positive staining for (B) Slit2, (C) Robo4 proteins and (D) Factor VIII-related antigen, but not (E) Robo1. (F) Negative control (immunocytochemistry performed without a primary antibody). Scale bar, 100 µm. Slit2, slit guidance ligand 2; Robo, roundabout guidance receptor; CECs, choroidal endothelial cells.