Table I.

Primer pairs for reverse transcription-quantitative polymerase chain reaction.

Gene	Primer sequence (5′-3′)	Annealing temperature (°C)
NGF	F: TGACTCCAAGCACTGGAACTCAT	60
	R: GTTTGTCGTCTGTTGTCAACGC
BDNF	F: GATGAGGACCAGAAGGTTCG	65
	R: GATTGGGTAGTTCGGCATTG
TNF-α	F: AAATGGGCTCCCTCTCATCAGTTC	58
	R: TCTGCTTGGTGGTTTGCTACGAC
IL-6	F: TCAACTCCATCTGCCCTTCAG	58
	R: AAGGCAACTGGCTGGAAGTCT
IL-1β	F: CACCTCTCAAGCAGAGCACAG	60
	R: GGGTTCCATGGTGAAGTCAAC
NPY	F: TGTCTCAGGGCTGGATCTCT	59
	R: TACTCCGCTCTGCGACACTA
POMC	F: GCTTCATGACCTCCGAGAAG	59
	R: TCTTGATGATGGCGTTCTTG
Ghrelin	F: AGCCCAGCAGAGAAAGGAAT	59
	R: GTGGCTGCAGTTTAGCTGGT
β-actin	F: AAGTCCCTCACCCTCCCAAAAG	58
	R: AAGCAATGCTGTCACCTTCCC

NGF, nerve growth factor; BDNF, brain-derived neurotrophic factor; TNF-α, tumor necrosis factor α; IL-6, interleukin 6; IL-1β, interleukin 1β; NPY, neuropeptide Y; and POMC, proopiomelanocortin.