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Results of low-copy plasma standards used for calculating the 95% LoD of iSCA v2.0
| HIV-1 RNA plasma standard (cps/ml) | No. of replicates tested | No. of replicates detected | Replicates detected (%) | Mean HIV-1 RNA cps expected | Mean HIV-1 RNA cps detected | Mean HIV-1 RNA (cps/ml) |
|---|---|---|---|---|---|---|
| 4 | 10 | 10 | 100 | 20 | 23.7 | 4.7 |
| 3 | 10 | 10 | 100 | 15 | 19.0 | 3.8 |
| 2 | 10 | 10 | 100 | 10 | 11.6 | 2.3 |
| 1 | 20 | 19 | 95 | 5 | 6.4 | 1.3 |
| 0.3 | 20 | 12 | 60 | 1.5 | 3.8 | 1.2 |
| 0.1 | 20 | 4 | 20 | 0.5 | 0.52 | 0.5 |
