FIG 2.

Analysis of the effect of depletion of anti-prM/E antibodies on NS1-MAC-ELISA using ZIKV VLP alone or in combination with VLPs from DENV-2, DENV-3, and WNV. (A) P/N ratios of IgM from ZIKV-infected patient serum 1 (primary Zika infection patient ID 48B) and 2 (secondary Zika infection patient ID 45B) using ZIKV VLP alone for depletion and ZIKV-NS1 antigens for NS1-MAC-ELISA (“+” on the x axis indicates depletion, and “–” indicates no depletion). (B) P/N ratios of IgM from one ZIKV-infected (black bar) or DENV-infected (white bar) patient serum using a single, double, triple, or quadruple combination of VLP antigens of ZIKV, DENV-2, DENV-3, and WNV for depletion, as indicated on the x axis. Normal human serum was used as a negative control to calculate the P/N ratio by dividing the OD₄₅₀ of ZIKV- or DENV-confirmed patient serum by that of the negative-control serum. All data were obtained based on triplicate results from three independent experiments, and the standard deviations are indicated. **, P < 0.0001 (P values were calculated using a two-tailed Student t test).