HeLa cells were co-transfected with miR-130a inhibitor and TIMP2 siRNA, or with miR-130a inhibitor and NC siRNA. (A) reverse transcription-quantitative polymerase chain reaction and (B) western blot analysis were performed to examine the mRNA and protein expression of TIMP2, respectively. (C) A wound healing assay (magnification, ×40) and (D) a Transwell assay (magnification, ×400) were performed to examine cell migration and invasion, respectively. **P<0.01 vs. miR-130a inhibitor+NC siRNA. siRNA, small interfering RNA; NC, negative control; TIMP, tissue inhibitor of metalloproteinases; miR, microRNA.