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. 2019 Mar 5;2019:3904923. doi: 10.1155/2019/3904923

Figure 3.

Figure 3

PTHrP is a direct target of miR-15a-5p. (a) Sequences of miR-15a-5p and potential miR-15a-5p-binding sites in the 3′UTR of PTHrP. (b) Luciferase activity assay for pGL3-PTHrP-wt or pGL3-PTHrP-mut versus the activity of Renilla luciferase in HEK293T cells after transient transfection with miR-NC or miR-15a-5p mimics (∗∗p<0.001). (c) Level of PTHrP in OA chondrocytes treated with miR-15a-5p-mimics or anti-miR-15a-5p by western blot (∗∗p<0.001). (d, e) RT-PCR analysis of the relative expression of miR-15a-5p or PTHrP (∗p<0.05, ∗∗p<0.001, n(normal) = 8, and n(OA) = 16). (f) Correlation between miR-15a-5p and PTHrP in OA chondrocytes.