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SIRT1 silencing inhibits epithelial-mesenchymal transition in U251 cells. The (A) mRNA and (B) protein levels of E-cadherin, β-catenin, fibronectin and vimentin in U251 cells following transfection with SIRT1-siRNA were determined using the reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. ***P<0.001. siRNA, silencing RNA; SIRT1, silent information regulator 1; E-cadherin, epithelial cadherin.
