Flow cytometry assessment of surface phenotypes for MNCs and KCs, nonactivated and activated in either M1- or M2-direction. (a) Flow cytometry assessment of surface phenotypes for nonactivated KCs, (b) flow cytometry assessment of surface phenotypes for nonactivated MNCs, (c) flow cytometry assessment of CD86 marker for MNCs and KCs, nonactivated and activated in either M1- or M2-direction, and (d) flow cytometry assessment of CD163 marker for MNCs and KCs, nonactivated and activated in either M1- or M2-direction. ∗p < 0.05 MNCs (M0) versus KCs (M0), CD86+MNCs (M2) versus CD86+KCs (M2), #p < 0.05 CD163+KCs (M0) versus CD163+KCs (M1), ## p < 0.05 CD86+ MNCs (M0) versus CD86+ MNCs (M1) and CD86+ MNCs (M2), and ### p < 0.05 CD163+ MNCs (M0) versus CD86+ MNCs (M1) and CD86+ MNCs (M2); KCs: Kupffer cells; MNCs: macrophages of monocytic lineage.