Figure 6.

The immunogenic potential of DBD plasma is not due to pulsed electric field and RONS alone, as evaluated using the vaccination assay. A) Syngeneic C57BL/6J mice were vaccinated once and challenged with 10⁴ live B16F10 melanoma cells on the following week (n = 8 per group). Tumor volumes were monitored for both B) the vaccination site and C) the challenge site. A dotted line was placed in the graphs on day 7, representing the day of live tumor challenge, while a dashed line was placed on day 50 to represent the predefined end of the study when survival was assessed. All remaining mice were sacrificed together when the final mouse with a tumor reached humane endpoints. D) Mouse survival was plotted for the entire predefined duration of the study (50 d) and the curves were compared with the log‐rank (Mantel–Cox) test. E) Tumor development on both flanks of the mice and percent protection against challenge tumors are listed here.