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. 2019 Feb 4;17(4):2666–2674. doi: 10.3892/etm.2019.7242

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Copyright: © Zhong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Protective effect of EVA on the Bcl-2/Bax ratio and caspase-3 activity in PC12 cells. Cells were incubated in the absence of Aβ_1-42 or in the presence of 20 µM Aβ_1-42 following pretreatment with EVA. (A) Levels of cleaved caspase-3, Bax and Bcl-2 were observed under a microscope (6 fields of view; magnification, ×400). Semiquantitative image analysis of (B) cleaved caspase-3 levels, and (C) Bcl-2/Bax ratio. Data are presented as the percentage of the control group and expressed as the mean ± standard deviation (n=6). #P<0.01 compared with the control group untreated with Aβ_1-42. *P<0.05 compared with the Aβ_1-42-treated group. EVA, ethyl vanillin; Aβ, β-amyloid; Bax, apoptosis regulator Bax; Bcl-2, apoptosis regulator Bcl-2.