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. Author manuscript; available in PMC: 2019 Mar 20.
Published in final edited form as: J Alzheimers Dis. 2019;67(1):393–410. doi: 10.3233/JAD-180941

Figure 4. TRMPL1 is involved in ML-SA-induced endolysosome acidification.

Figure 4.

(A) Ratiometric endolysosome pH trace shows the effect of ML-SA1 (20 μM) on endolysosome pH in primary cultured rat cortical neurons. Quantified data shows that ML-SA1 (20 μM) decreased significantly endolysosome pH (2 min following ML-SA1 treatment vs baseline, n=19 neurons of 4 different plates from two different animals). (B) Immunoblotting shows that TRPML1 siRNA decreased significantly protein levels of TRPML1 in primary cultured neurons (n=4). (C) Basal levels of endolysosome pH did not change following treatment withTRPML1 siRNA; however, the magnitude of ML-SA1-induced acidification of endolysosomes (2 min following ML-SA1 treatment) was decreased in TRPML1 siRNA treated neurons when compared with negative siRNA treated neurons (*p<0.05, n=15–20 neurons of 4 different plates from two different animals).