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. Author manuscript; available in PMC: 2019 Mar 20.
Published in final edited form as: Biologicals. 2016 Apr 8;44(4):191–197. doi: 10.1016/j.biologicals.2016.02.005

Fig. 1. Cation-exchange chromatography from A. c. pictigaster venom.

Fig. 1.

Five hundred microliters of A. c. pictigaster venom (60 mg/mL) was injected to a cationic exchange HPLC column Waters™ SP 5PW (75 × 7.5 mm). The fractions were separated with a 0.02 M sodium phosphate buffer, pH 6.2, containing 0.5 M NaCl. The separation required 60 minutes with a flow rate of 1.0 mL/min. The absorbance was measured at 280 nm by a Waters™ 2487 Dual absorbance detector. The proteolytic activity was tested (hemorrhagic, fibrinolytic, and gelatinase) for each fraction.