Skip to main content
. Author manuscript; available in PMC: 2019 Jul 25.
Published in final edited form as: J Am Chem Soc. 2018 Jul 16;140(29):9108–9119. doi: 10.1021/jacs.8b02129

Figure 5.

Figure 5.

(A) Thermograms and representative ITC-derived Zn binding curves obtained for L34A CzrA homodimers at 15.0, 25.0, and 35.0 °C from left to right (data for the other variants are presented in Figure S13). (B) Temperature-dependence of the ΔH of binding of Zn to the CzrA homodimer in steps. Binding of the first Zn(II) (left) and binding of the second Zn (right) to the homodimer. The continuous lines are linear fits that define the slope ΔCp = dΔH/ dT for each variant.