qPCR reveals no significant differences among reproductively relevant hypothalamic and pituitary genes. (A) Arcuate micropunches show no differences between WT and Bmal1 KO expression of Dynorphin or Kiss1R (t test, n = 4 to 5; Kiss1R, P = 0.05). (B) AVPV micropunches show no significant differences between WT and Bmal1 KO expression of V1a or tyrosine hydroxylase (TH; t test, n= 4 to 5). (C) POA micropunches show no differences between WT and Bmal1 KO expression of Kiss1R or GnRH (t test, n = 5). (D) Whole pituitary shows no significant differences among FSHβ, LHβ, GnRH-R, and Kiss1R (t test, n = 3 to 4; Kiss1R, P > 0.05).