Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 13;3(4):716–733. doi: 10.1210/js.2018-00228

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Endocrine Society

This article has been published under the terms of the Creative Commons Attribution Non-Commercial, No-Derivatives License (CC BY-NC-ND; https://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Figure 5. — Validation of conditional KO in GnRH-Bmal1^−/− and Kiss-Bmal1^−/− mice. (Ai) GnRH (magenta), (Aii) BMAL1 (green), and (Aiii) merged image with DAPI (blue) from a Bmal1^fl/fl mouse. GnRH neurons are identified by arrowheads in the isolated and merged images. (Bi) GnRH (magenta), (Bii) BMAL1 (green), and (Biii) merged image of with DAPI (blue) from a GnRH-Bmal1^−/− mouse. (C) Quantification of the percentage of GnRH and Bmal1 colocalization in Bmal1^fl/fl and GnRH-Bmal1^−/− mice (t test, n = 3 to 4; P < 0.003). (D) Representative PCR product from genomic DNA of micropunches showing Bmal1 recombination in the AVPV and ARC of the Kiss-Bmal1^−/− mouse, but not in regions lacking Kiss1 expression (~0.57-kb band). Recombination in a Bmal1 KO tail sample is shown as a positive control. ***P < 0.001. Cere., cerebellum; NTC, no template control; Thal., thalamus.