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. 2019 Feb 13;3(4):716–733. doi: 10.1210/js.2018-00228

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Copyright © 2019 Endocrine Society

This article has been published under the terms of the Creative Commons Attribution Non-Commercial, No-Derivatives License (CC BY-NC-ND; https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Figure 6. — Bmal1 KO in GnRH or Kiss1 neurons does not affect pubertal onset or estrous cycling. (A) Age (d) and (B) weight (g) of vaginal opening in Bmal1^fl/fl, GnRH-Bmal1^−/−, and Kiss-Bmal1^−/− female mice (n = 3 to 6; one-way ANOVA not significant). (C) Percentage of time spent in each stage during 25 consecutive days of vaginal cytology (n = 7 to 13, two-way ANOVA significant for effect of stage; F_2,51 = 52.15, P < 0.0001). (D) Average cycle length across 25 consecutive days of vaginal cytology (n = 7 to 13; one-way ANOVA not significant). (E) Number of Graafian follicles per section observed in ovaries in light/dark (LD) and constant darkness (DD) conditions (n = 2 to 15, two-way ANOVA significant for effect of lighting; F_1,24 = 24.42, P < 0.0001; Sidak post hoc multiple comparisons test). (F) Number of corpora lutea observed per section in LD and DD conditions (n = 2 to 15; two-way ANOVA not significant). **P < 0.01, ***P < 0.001.