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. 2019 Feb 13;3(4):716–733. doi: 10.1210/js.2018-00228

Table 1.

Primers for qPCR

Gene Forward (5′→3′) Reverse (5′→3′)
H2afz TCACCGCAGAGGTACTTGAG GATGTGTGGGATGACACCA
Ppia AAGTTCCAAAGACAGCAGAAAAC CTCAAATTTCTCTCCGTAGATGG
cFos GGCAAAGTAGAGCAGCTATCTCCT TCAGCTCCCTCCTCCGATTC
Kiss1 TGCTGCTTCTCCTCTGT ACCGCGATTCCTTTTCC
GnRH ACACTTGGTTGAGTCTTTCCA TGGCTTCCTCTTCAATCAGAC
V1a AGCGCCTACATCCTCTGCTG AGTAACGCCGTGATCGTGGT
Dynorphin GTGTGCAGTGAGGATTCAGG AGTCATCCTTGCCACGGAGC
Kiss1R GACCATGCAGACAGTTACC GCAGCACAGTAGGAAAGTGAC
TH CAGCCCTACCAAGATCAAAC GTGTACGGGTCAAACTTCA
FSHβ GCCGTTTCTGCATAAGC CAATCTTACGGTCTCGTATACC
LHβ CTGTCAACGCAACTCTGG ACAGGAGGCAAAGCAGC
GnRH-R GCCCCTTGCTGTACAAAGC CCGTCTGCTAGGTAGATCATCC

Abbreviation: TH, tyrosine hydroxylase.