Fig. 11.

Depletion of BMPR2 from macrophages in fibrosis. Isolated bone-marrow-derived macrophages (BMDMs) from BLM-exposed mice treated with PBS, IL6 or IL6+IL6R to induce trans-signaling (trans) and subsequent immunoblots for BMPR2, PSTAT3, ARG-1, CD206, and β-actin expression levels (A); or transcript levels for Col1A1 (B), Col1A2 (C), and IL6 (D). *P ≤ 0.05, ANOVA comparisons between PBS and IL6 or IL6+ILR groups. Immunohistochemistry for BMPR2 (green signals), CD206 (red signals), merge in yellow and counterstain with DAPI from lung sections from mice exposed to PBS or BLM (E) or from human normal, IPF, and IPF+PH lung sections (F). White arrowheads denote macrophages; scale bar = 50 μm.