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. 2019 Mar 20;19:109. doi: 10.1186/s12870-019-1715-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — PCR-based identification of T-DNA-free T₁ segregants using primers directed at the hpt sequence. Segregants among (a) tms5–1 plants, and (b) tms5–2 plants. VK005–01 was used as the positive control (CK+) and cv. YK17 as the negative control (CK-). M: 2 kbp DNA ladder