Fig. 5.

Gene expression in fetal pancreas and islet. Genes were assessed in total pancreas from male and female reduced uterine perfusion pressure (RUPP) and dams using either β-actin (A–F, n = 3 per sex), Rplp0 (A′–E′, n = 3 per sex) as internal housekeeping gene, or in isolated islets [A′′–E′′, G, H, where n = 3 pseudoreplicates from a pool of 10–12 fetal pancreata (n = 3 litters of Sham or RUPP)]. Ctrl, control (sham-operated). Asterisks indicate significance RUPP vs. Ctrl by using a mixed model ANOVA with random litter effects. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.