Table 3.

Effects of different substances and incubation conditions on the inhibitory activity of the cell free supernatant of bacteriocinogenic lactic acid bacteria isolated from calabresa against L. monocytogenes 72

effect	substance/condition	concentration	producer strain
			L. curvatus 12	L. curvatus 36	W. viridescens 23
Enzymesa	trypsin	0.1 mg/mL	AI	AI	AI
	proteinase K	0.1 mg/mL	AI	AI	AI
	papain	0.1 mg/mL	AI	AI	AI
	pepsin	0.1 mg/mL	AI	AI	AI
	protease	1 mg/mL	AI	AI	AI
	α-amilase	1 mg/mL	I	I	I
	lipase	1 mg/mL	I	I	I
	catalase	1 mg/mL	I	I	I
pHa	3		AI	AI	I
	5		AI	AI	I
	7		I	I	I
	8		I	AI	I
	10		I	AI	I
Temperatureb	7 °C for 30 min		3200	12,800	25,600
	25 °C for 30 min		3200	25,600	25,600
	37 °C for 30 min		25,600	25,600	25,600
	40 °C for 30 min		25,600	12,800	25,600
	60 °C for 30 min		25,600	12,800	25,600
	80 °C for 30 min		25,600	6400	25,600
Chemicalsb	NaCl	10 mg/mL	3200	0	200
	EDTA	10 mg/mL	200	800	800
	Tween 80	10 mg/mL	200	0	400

^aeffect of enzymes was assessed by the spot-on-the-lawn method. “AI” indicates absence of inhibition, “I” indicates inhibition; ^b effects of pH, temperature and chemicals were assessed by a quantitative assay, and results expressed as arbitrary units per mL