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. 2019 Mar 20;19:63. doi: 10.1186/s12866-019-1436-4

Table 4.

Effects of variations on MRS broth on inhibitory activity of bacteriocinogenic lactic acid bacteria isolated from calabresa against L. monocytogenes 72, assessed by a quantitative assay

Effect MRS variant Concentration (mg/mL) Bacteriocinogenic strain
L. curvatus 12 L. curvatus 36 W. viridescens 23
Control MRS 25,600 25,600 25,600
pH 2 200 0 1600
4 25,600 200 1600
6 25,600 25,600 25,600
8 1600 0 1600
10 12,800 0 0
12 25,600 0 0
Carbo-hydrate lactose 20 1600 0 25,600
sacarose 20 800 0 25,600
mannitol 20 3200 0 800
fructose 20 25,600 0 25,600
dextrose 20 25,600 25,600 25,600
maltose 20 25,600 0 25,600
raffinose 20 12,800 100 25,600
Organic nitrogen peptone 25 25,600 0 25,600
meat extract 25 25,600 0 25,600
yeast extract 25 25,600 100 25,600
peptone + meat extract 12.5 + 12.5 25,600 100 12,800
peptone + yeast extract 15 + 7.5 25,600 200 25,600
meat extract + yeast extract 15 + 7.5 25,600 100 25,600
peptone + meat extract + yeast extract 10 + 10 + 5 25,600 25,600 25,600
Chemicals KH2PO4 0 25,600 100 12,800
2 25,600 25,600 25,600
5 25,600 100 25,600
10 25,600 0 25,600
MgSO4 0 25,600 25,600 100
0.1 25,600 25,600 25,600
0.5 25,600 25,600 100
MnSO4 0 1600 25,600 0
0.05 25,600 25,600 25,600
0.2 25,600 0 100
Sodium acetate 0 25,600 0 25,600
5 25,600 25,600 25,600
10 25,600 100 25,600
Tri-ammonium citrate 0 25,600 0 25,600
2 25,600 25,600 25,600
5 25,600 100 25,600
Tween 80 0 12,800 0 12,800
1 25,600 25,600 25,600
2 25,600 100 25,600
5 25,600 200 25,600