Table 1.
The efficiency of DPC formation upon oxidation of 8-oxodGuo in NCPs.
| DNA sequence | H4 mutants in NCP | Oxidation%[a] | DPC% | EDPC[b] |
|---|---|---|---|---|
| 8-oxodGuo89/C | WT [c] | 31 ± 4 | 30 ± 1 | 0.97 |
| Del 1–20 [d] | 12 ± 5 | 4 ± 1 | 0.33 | |
| K5,8,12,16,20R [e] | 37 ± 6 | 38 ± 1 | 1 | |
| S1A-K5,8,12,16,20R[f] | 28 ± 8 | 22 ± 1 | 0.78 | |
| S1A-K5,8,12,16,20R-H18A [g] | 41 ± 10 | 25 ± 1 | 0.61 | |
| CapN [h] | 35 ± 2 | 6 ± 1 | 0.17 | |
| CapN- K5,8,12,16,20R[i] | 28 ± 3 | 6 ± 2 | 0.21 | |
| 8-oxodGuo89/A | WT | 46 ± 3 | 50 ± 1 | 1 |
| 8-oxodGuo89/T | WT | 57 ± 4 | 54 ± 3 | 0.95 |
| 8-oxodGuo73/C | WT | 48 ± 5 | 6 ± 1 | 0.13 |
| 8-oxodGuo137/C | WT | 35 ± 12 | 18 ± 3 | 0.51 |
[a]
oxidation% is the initial 8-oxo-dGuo oxidation yield (after 10 minutes incubation using 10 mM K3Fe(CN)6). Yields are averages ± standard deviations of at least two experiments.
[b]
EDPC = DPC%/oxidation%.
[c]
Wild-type histone H4.
[d]
Histone H4 without the N-terminal 1−20 amino acids. The sequence of N-terminal tail of is SGRGKGGKGLGKGGAKRHRK.
[e]
H4 with Lys 5, 8, 12, 16, 20 to Arg mutations.
[f]
H4 with Ser 1 to Ala and Lys 5, 8, 12, 16, 20 to Arg mutations.
[g]
H4 with Ser 1 to Ala, Lys 5, 8, 12, 16, 20 to Arg and His 18 to Ala mutations.
[h]
H4 with capped N-terminal by thiazolidine.
[i]
H4 with both capped N-terminal and Lys 5, 8, 12, 16, 20 to Arg mutations.