Fig. 2.

Clinical markers of GI toxicity. a Rats treated with MTX developed severe diarrhea from 48 h. b Severe diarrhea was not seen until 72 h in rats treated with MTX + AZD3342. AZD3342 did not reduce the severity of MTX-induced diarrhea from 72 h. AZD3342 did not prevent MTX-induced weight loss (c) or intestinal barrier dysfunction (d). Diarrhea data are shown as percentage of animals experiencing grade 0–3 diarrhea. Weight loss and serum FITC-dextran data are shown as the mean ± SEM and were analyzed using a two-way ANOVA with Tukey's post hoc test.