Fig. 3.

Intestinal injury assessment. a Representative photomicrographs of the jejunum (original magnification, ×100). Arrows indicate crypt loss (filled arrows), surface enterocyte loss and villous blunting (open arrows), and inflammatory infiltrate (arrowhead). b Small intestinal wet weights indicate MTX-induced atrophy. AZ3342 did not prevent MTX-induced small intestinal atrophy. c Histological damage scoring in the jejunum shows a significant increase in injury in groups treated with MTX. AZD3342 did not prevent MTX-induced injury (p = ns). No changes were seen in tissue injury scores for the colon (data not shown). Data are shown as the mean ± SEM; * p < 0.05 versus vehicle control, ^# p < 0.05 versus AZD3342 control. All data were analyzed using a two-way ANOVA with Tukey's post hoc test.