Tumor growth over 120 h. a MTX and MTX + AZD3342 inhibited tumor size compared to vehicle control and AZD3342 alone. A significant reduction in tumor size was seen at 72 h between AZD3342 alone and vehicle control (** p = 0.003). b Reduced tumor weight was seen in rats treated with MTX and MTX + AZD3342 compared to vehicle control but no differences between MTX and MTX + AZD3342 were found. This effect was seen at 72 h (MTX vs. vehicle, * p = 0.001; MTX + AZD3342 vs. vehicle, * p = 0.0003), 96 h (MTX vs. vehicle, * p < 0.0001; MTX vs. AZD3342, #
p = 0.0002; MTX + AZD3342 vs. vehicle, * p < 0.0001; MTX + AZD3342 vs. AZD3342, #
p < 0.0001), and 120 h (MTX vs. vehicle, * p < 0.0001; MTX vs. AZD3342, #
p < 0.0001; MTX + AZD3342 vs. vehicle, * p < 0.0001; MTX + AZD3342 vs. AZD3342, #
p < 0.0001). All data were analyzed using a two-way ANOVA with Tukey's post hoc test. Data are presented as the mean ± SEM.