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Graphical summary of the pipeline of work. (A) Summary of the different steps of in silico work designing the donors, the sgRNA and the oligonucleotides used to generate the constructs. (B) Graphical summary of the different steps involved in the generation of the donor and sgRNA construct. (C) Different paths of the knock-in and removal of the construct based on a fluorescent guided picking or FACS panclone generation approach.
