| Reagent | Volume for 1 reaction |
|---|---|
| Genomic DNA | Volume of 100–200 ng |
| GXL polymerase (10U/μl) | 1 μl |
| GXL polymerase buffer (5x) | 10 μl |
| dNTPs (2.5mM) | 4 μl |
| Primer forward (10 μM) | 2 μl |
| Primer reverse (10 μM) | 2 μl |
| H20 | to 50 μl |
| Total | 50 μl |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.