Fig. 6.

Box plots for average staining index (SI) scores evaluating immunohistological analysis (IHC) of primary mammary tumors derived from MDA-MB-231-Luc-D3H1 xenographs for saline and SFN groups. Each IHC staining was examined by two independent experts. The average SI scores for control and SFN treated groups were analyzed by T-test (same as ANOVA in this case since there are two groups [SFN treated group and control] and two sets of SI scores). The analysis resulted in a p-value of 0.08 with a negative 1.3 fold change for SFN vs. saline indicating that SFN down-regulated onco-protein Cripto expression by 30%. These results show that SFN consistently down-regulates Cripto protein expression in vivo. At least eight areas of 20x microscope fields were examined per sample. SFN: Sulforaphane.