Figure 6.

Left panel: theoretical correlation of the Huggins constant k_H, Eq. (21), and the second virial coefficient B₂₂, for the conditions of Fig. 3 and Fig. S1 – colors from cold to hot correspond to different temperatures. Note that both quantities are valid for dilute protein solutions, in our case this is up to 90 mg/mL. Right panel: correlation between the viscosities and second virial coefficients, B₂₂, at T = 20 °C and γ = 150 mg/mL. (i) η–B₂₂ theoretical correlation for antistreptavidin IgG₁ solutions studied above: viscosities ( ) correspond to fits in Fig. 3 and Fig. S1 and are correlated with B₂₂ values from the left panel for solutions with different pH and concentration of NaCl. The green line is the best least–square fit through the data. (ii) experimentally measured correlation of Saito et al [59] for mAb–A (●), mAb–B (×), and mAb–C (Δ) solutions. Black lines serve to guide the eye.