FIGURE 3.

Unilateral peripheral nerve injury alters Luman levels detected in axotomized ipsilateral dorsal root ganglion (DRG) neurons. Left Column: Representative immunofluorescence photomicrographs of L5 DRG sections (6 μm) detecting Luman protein in DRG ipsilateral to L4-L6 spinal nerve transection rats were at 1-hour, 1-day, 2-day, 4-day and 1-week post-injury. Scale bar = 50 μm. Naïve animals served as controls. Sections shown were all mounted on the same slide to ensure processing under identical conditions and are those for which the quantification is shown Right column: Representative scatterplots depicting relative changes in Luman immunofluorescence signal over individual neuronal cytoplasmic and nuclear regions as related to neuron size. Experimental states as indicated. Dashed lines divide the plots into low versus moderate to heavily labeled populations (n = 181–206 neurons analyzed per condition/animal). A total of 3 separate animals were analyzed in this manner per condition.