Fig 3. Effects of ethanol (EtOH), acetaldehyde (AcH) and phosphatidylethanol (PEth) on other vasoconstrictor effects and myogenic tone in mesenteric resistance arteries.
Pressure myography experiments with arteries pressurized to 80 mmHg. Data are mean ± SEM. A. aVP-induced constriction in % of the outer diameter (o.d.) in arteries pretreated with single doses of EtOH for 30 min, as in Fig 1. Vehicle—n = 5; 17.4 mM EtOH–n = 5; 50 mM EtOH–n = 4; 100 mM EtOH–n = 5. B. EC50 concentrations for the vasoconstrictor effects of aVP (nM) from dose-response curves in A. *: p<0.05 vs. vehicle. C. aVP-induced constriction in % of the o.d. in arteries pretreated with AcH (10 μM, n = 6) or PEth (2.1 μM, n = 4) for 30 min. Vehicle–n = 6. D. Endothelin-1-induced constriction in % of the o.d. in arteries pretreated with EtOH (100 mM, n = 5), AcH (10 μM, n = 3) or PEth (2.1 μM, n = 3) for 30 min. Vehicle–n = 7. E. KCl-induced constriction in % of the o.d. in arteries pretreated with EtOH (100 mM, n = 3), AcH (10 μM, n = 3) or PEth (2.1 μM, n = 3) for 30 min. F. Effects of EtOH (100 mM, n = 3), AcH (10 μM, n = 3) and PEth (2.1 μM, n = 3) on myogenic tone. Vehicle–n = 3. Arteries were pretreated for 30 min. Data are expressed as % change of o.d. when arteries were exposed to various pressures (100% = o.d. at 80 mmHg).