Fig 4. Flux comparisons of mutated model and wild type for 2,3-butanediol overproduction.

This figure shows the main path of 2,3-butanediol production in yeast and effective reactions identified by OptRAM. Solid arrows indicate the direction of metabolic reactions. Red arrows indicate that the fluxes are predicted to be higher in the mutated strain and green arrows indicate the flux is predicted to be lower than in wildtype. Gray arrows indicate the reactions are not significantly different between the designed strain and the wildtype. Green dotted boxes highlight the critical down-regulated reactions.