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. 2018 Jan 1;24(1-2):34–46. doi: 10.1089/ten.tea.2017.0011

FIG. 3.

FIG. 3.

The effect of ECM and mechanical stimulation upon the macrophage secretome. Macrophages were treated with activating cytokines IFNy+ LPS or IL-4, or 200 μg/mL of ECM degradation products for 18 h. After 18 h, cells were washed and media were replaced with serum-free, ECM-free media. Macrophages were strained for 5 h and their conditioned media were collected for C2C12 experiments. (A, D) IL-4-treated and ECM-treated macrophages promote increased C2C12 migration. Mechanically loaded macrophages significantly increase C2C12 migration compared to the unloaded control macrophages and the low-serum negative control. (B, E) IFNy+LPS-treated macrophages promote increased C2C12 mitogenesis. Mechanically loaded macrophages significantly increase C2C12 mitogenesis compared to the unloaded and proliferation media positive control. (C, F) IL-4-stimulated and M-ECM-stimulated macrophages significantly increase C2C12 myogenesis. Mechanically loaded macrophages significantly increase C2C12 myogenesis compared to the unloaded and proliferation media negative control. (#p < 0.05 when compared to the 20% FBS control, *p < 0.05 compared to the indicated groups, ***p < 0.01 when compared to the 20% FBS and IFNy+LPS groups, n = 5, error bars represent standard error of the mean). FBS, fetal bovine serum.