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. 2019 Apr;25(4):496–506. doi: 10.1261/rna.067967.118

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© 2019 Paul et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Effect of the expression of amino-terminal fragments of Bcd1p on cell growth, on selected snoRNA steady-state expression levels and association with selected snoRNAs. (A) The GAL1::3HA-BCD1 strain was transformed with a p416 empty vector or p416 vectors expressing Flag-tagged versions of Bcd1p, either full length or amino-terminal fragments. The cells were maintained in YPG medium, then shifted to YPD medium at an OD_600nm of 0.05 for 15 h, and their growth was monitored. (B) The relative RNA content of the same cells was analyzed when maintained in YPG medium after a 16-h shift to YPD medium. The ALG9 gene was used as an endogenous control. (C) RIP Bcd1p assays were performed on the same cells cultivated in repressive YPD medium for 16 h. The IP was performed with anti-Flag beads, and signal levels obtained in cells transformed with an empty p416 vector were set to 1.